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KMID : 0903520050480040425
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 4 p.425 ~ p.430
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Production of β-sitosterol by Cell Suspension Culture of Chrysanthemum coronarium L.
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Kim Hyun-Chul
Chung Ha-Young
Lee So-Yeon
Chung Ho-Yong
Kim You-Jung
Baek Nam-In
Kim Sung-Hoon
Choi Geun-Won
Kim Dae-Keun
Kwon Byoung-Mog
Park Mi-Hyun
Chung In-Sik
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Abstract
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β-sitosterol is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for β-sitosterol production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal β-sitosterol production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l KNO3, 1650 mg/l NH4NO3), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of β-sitosterol was 150 μg/g DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum β-sitosterol content of 143.8 μg/g DCW was obtained at an air-flow rate of 100 cc/min.
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KEYWORD
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β-sitosterol, callus, suspension culture, air-lift bioreactor
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